Protein Molarity Calculator Neb

Protein Molarity Formula:

\[ M (\mu M) = \frac{Conc (mg/mL)}{MW (kDa)} \times 1000 \]

Molarity (M):

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1. What is the Protein Molarity Calculator?

The Protein Molarity Calculator converts protein concentration from mass/volume (mg/mL) to molarity (µM) using the protein's molecular weight. This is essential for accurate experimental setup and reproducibility in molecular biology research.

2. How Does the Calculator Work?

The calculator uses the NEB protein molarity formula:

\[ M (\mu M) = \frac{Conc (mg/mL)}{MW (kDa)} \times 1000 \]

Where:

\( M \) — Molarity in micromolar (µM)
\( Conc \) — Protein concentration in milligrams per milliliter (mg/mL)
\( MW \) — Molecular weight in kilodaltons (kDa)

Explanation: The formula converts mass concentration to molar concentration by dividing by molecular weight and multiplying by 1000 to convert to micromolar units.

3. Importance of Protein Molarity Calculation

Details: Accurate molarity calculation is crucial for proper experimental design, ensuring correct stoichiometry in reactions, and maintaining reproducibility across experiments.

4. Using the Calculator

Tips: Enter protein concentration in mg/mL and molecular weight in kDa. Both values must be positive numbers. The calculator will provide the molarity in µM.

5. Frequently Asked Questions (FAQ)

Q1: Why convert to molarity instead of using mass concentration?
A: Molarity allows for accurate stoichiometric calculations and ensures consistent molar ratios between different proteins and reagents in experiments.

Q2: How do I determine the molecular weight of my protein?
A: Molecular weight can be calculated from the amino acid sequence using bioinformatics tools or obtained from protein databases and literature.

Q3: What if my protein has multiple subunits?
A: Use the molecular weight of the functional unit (the complete multimeric complex) for accurate molarity calculations.

Q4: Are there limitations to this calculation?
A: This calculation assumes the protein is pure and the concentration measurement is accurate. Post-translational modifications may affect the actual molecular weight.

Q5: Can this calculator be used for other biomolecules?
A: While the formula is generally applicable, this calculator is specifically designed for proteins and uses kDa as the molecular weight unit.