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Protein Molar Weight Calculation:
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Protein molar weight (molecular weight) is the mass of one mole of a protein molecule, typically expressed in grams per mole (g/mol). It's calculated by summing the atomic weights of all atoms in the molecule.
The calculator uses the formula:
Where:
Explanation: The calculator sums the molecular weights of all amino acids in the protein sequence, accounting for the water molecule removed during peptide bond formation.
Details: Knowing a protein's molecular weight is essential for various applications including protein purification, gel electrophoresis, mass spectrometry, biochemical assays, and pharmaceutical development.
Tips: Enter the protein amino acid sequence using single-letter codes. The calculator will automatically ignore any non-amino acid characters and calculate the molecular weight.
Q1: Why does the calculation start with water molecular weight?
A: During protein synthesis, a water molecule is removed for each peptide bond formed. The calculation accounts for this.
Q2: Are post-translational modifications considered?
A: No, this calculator provides the theoretical molecular weight of the unmodified polypeptide chain.
Q3: What about disulfide bonds?
A: Disulfide bonds are not accounted for in this basic calculation as they don't change the molecular weight (just the structure).
Q4: How accurate is this calculation?
A: This provides a theoretical molecular weight based on average atomic masses. Actual measured values may vary slightly due to isotopic distributions.
Q5: Can I use three-letter amino acid codes?
A: No, this calculator only accepts single-letter amino acid codes (A, R, N, D, C, Q, E, G, H, I, L, K, M, F, P, S, T, W, Y, V).