1. What is Protein Molar Concentration?

Protein molar concentration represents the number of moles of a protein per liter of solution, typically expressed in nanomolar (nM) units. It's a fundamental measurement in biochemistry and molecular biology experiments.

2. How Does the Calculator Work?

The calculator uses the protein molar concentration formula:

Where:

• \( M \) — Molar concentration (nM)
• \( Conc \) — Mass concentration (ng/µL)
• \( MW \) — Molecular weight (kDa)

Explanation: This formula converts mass concentration to molar concentration by accounting for the molecular weight of the protein.

3. Importance of Molar Concentration Calculation

Details: Accurate molar concentration is essential for preparing consistent experimental conditions, determining stoichiometry in binding assays, and ensuring reproducibility in biochemical experiments.

4. Using the Calculator

Tips: Enter concentration in ng/µL and molecular weight in kDa. Both values must be positive numbers. The result will be displayed in nanomolar (nM) units.

5. Frequently Asked Questions (FAQ)

Q1: Why convert to molar concentration?
A: Molar concentration allows researchers to work with consistent molecular quantities regardless of protein size, enabling accurate comparisons between different proteins.

Q2: What's the difference between ng/µL and nM?
A: ng/µL is a mass concentration (weight/volume), while nM is a molar concentration (moles/volume). The conversion depends on the molecular weight.

Q3: How do I determine molecular weight?
A: Molecular weight can be calculated from the amino acid sequence or measured experimentally using techniques like mass spectrometry.

Q4: Are there limitations to this calculation?
A: This calculation assumes the protein is pure and monodisperse. Impurities or protein aggregates may affect accuracy.

Q5: Can I use this for other biomolecules?
A: Yes, this formula works for any molecule where you know the mass concentration and molecular weight.