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Bioline Protein Molarity Formula:
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The Bioline Protein Molarity Calculator converts protein concentration from micrograms per milliliter (µg/mL) to micromolar (µM) using the protein's molecular weight. This conversion is essential for standardizing protein concentrations in experimental protocols.
The calculator uses the protein molarity formula:
Where:
Explanation: The formula converts mass concentration to molar concentration by accounting for the molecular weight of the protein.
Details: Accurate protein molarity calculation is crucial for experimental consistency, reagent preparation, and ensuring proper stoichiometry in biochemical assays and reactions.
Tips: Enter protein concentration in µg/mL and molecular weight in kDa. Both values must be positive numbers. The calculator will output the molar concentration in µM.
Q1: Why convert protein concentration to molarity?
A: Molarity allows for standardized comparisons between different proteins and ensures consistent molar ratios in experiments.
Q2: What if my molecular weight is in Daltons instead of kDa?
A: Simply divide your molecular weight in Daltons by 1000 to convert to kDa before using the calculator.
Q3: Can this calculator be used for other biomolecules?
A: While designed for proteins, the same formula applies to any molecule where you need to convert mass concentration to molar concentration.
Q4: How accurate is this conversion?
A: The conversion is mathematically exact, but accuracy depends on the precision of your input values for concentration and molecular weight.
Q5: Why is the conversion factor 1000?
A: The factor 1000 accounts for the conversion between µg (10⁻⁶ g) and kDa (10³ g/mol), and mL to L in the concentration units.