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Protein Molarity Formula:
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The Bioline Protein Calculator converts protein concentration from weight/volume (µg/µL) to molarity (µM) using the protein's molecular weight. This conversion is essential for many biological experiments requiring precise molar concentrations.
The calculator uses the formula:
Where:
Explanation: The formula converts mass concentration to molar concentration by accounting for the molecular weight of the protein. The factor of 1000 converts between the different units of measurement.
Details: Accurate molarity calculation is crucial for experiments requiring specific molar ratios, such as enzyme kinetics studies, protein-protein interactions, and setting up reaction mixtures with precise stoichiometry.
Tips: Enter protein concentration in µg/µL and molecular weight in kDa. Both values must be positive numbers. For best results, use accurate molecular weight values from protein sequencing or manufacturer specifications.
Q1: Why convert to molarity instead of using weight/volume?
A: Molarity allows researchers to work with exact molecular ratios, which is essential for many biochemical experiments where stoichiometry matters.
Q2: How do I determine my protein's molecular weight?
A: Molecular weight can be determined through protein sequencing, mass spectrometry, or calculated from the amino acid sequence if known.
Q3: What if my protein has multiple subunits?
A: Use the molecular weight of the functional unit (holoprotein) for your calculations, not individual subunits.
Q4: Can this calculator be used for other biomolecules?
A: While designed for proteins, the same formula works for any molecule when you know its molecular weight.
Q5: Why is the result in micromolar (µM) units?
A: Micromolar concentrations are commonly used in biological experiments as they represent practical working concentrations for most proteins.